A display device as well as a method and a device for adjusting information channels of the same are disclosed. The adjusting method includes: turning the display device on; transmitting a picture to a display screen (1) of the display device for display, the picture comprising a plurality of sub-pictures in one-to-one correspondence with a plurality of sub-screens (11) which are spliced to form the display screen (1); acquiring an image displayed on the display screen (1); processing the acquired image, comparing the processed image with the picture, and identifying arrangement sequence of a plurality of information channels of the display device; and adjusting the respective information channels such that the image displayed by the display screen (1) is the same as the picture. The adjusting method compares the image displayed on the display screen of the display device with the original picture, identifies and adjusts the respective information channels, so there is no need for viewing the display system for confirmation every time the line sequence is adjusted, thereby achieving a quick and convenient adjustment.