A transillumination device having a yellow-orange light source, with LEDs having a wavelength in the range of 585-595 nm, preferably 590 nm, an amber light source with LEDs having a wavelength of light in the range of approximately 600-610 nm, preferably 605 nm, and a lime green LEDs light source having a wavelength in the range of approximately 535-545 nm, preferably 540 nm. Yellow-orange LED light sources have been found to be beneficial for transillumination of superficial veins, amber light sources have been found to be beneficial for transillumination of deeper veins and lime green light sources have been found to be beneficial for transillumination of arteries.