A multilayer film including: a first long-length substrate; and an optically anisotropic layer that is formed directly on the first substrate and contains cured liquid crystal molecules, wherein the first substrate has an Nz factor of 1.1 to 3.0, variation of orientation angle of 1.0° or less, and an orientation-regulating force caused by stretching, and an angle formed between a slow axis of the first substrate and a widthwise direction of the first substrate is 0° or more and less than 90°; and manufacturing method and use thereof.