Invention Grant
- Patent Title: Method for cultivating cells in adhesion culture by using a cell culture carrier in capsule form, and cell culture carrier therefor
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Application No.: US15033108Application Date: 2014-09-22
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Publication No.: US10208281B2Publication Date: 2019-02-19
- Inventor: Daniel Hans Rapoport , Miriam Voigt , Charlotte Walter
- Applicant: Fraunhofer-Gesellschaft zur Foerderung der angewandten Forschung e. V.
- Applicant Address: DE Munich
- Assignee: FRAUNHOFER-GESELLSCHAFT ZUR FOERDERUNG DER ANGEWANDTEN FORSCHUNG E.V.
- Current Assignee: FRAUNHOFER-GESELLSCHAFT ZUR FOERDERUNG DER ANGEWANDTEN FORSCHUNG E.V.
- Current Assignee Address: DE Munich
- Agency: Caesar Rivise, PC
- Priority: DE102013018242 20131030
- International Application: PCT/EP2014/002567 WO 20140922
- International Announcement: WO2015/062686 WO 20150507
- Main IPC: C12M1/12
- IPC: C12M1/12 ; C12N5/00

Abstract:
The invention relates to a method for cultivating cells in adhesion culture, comprising at least the following steps: a) dissolving or suspending a cross-linkable, biocompatible material having adhesion points for cells in a cell culture medium; b) suspending cells in the cell culture medium, which contains the cross-linkable, biocompatible material, or in a medium that contains at least one component that is required for the cross-linking of the cross-linkable, biocompatible material; c) introducing the cell suspension into a medium in drops under conditions that initiate or permit the cross-linking of the biocompatible material, wherein either the cell suspension or the medium into which the cell suspension is introduced in drops contains the cross-linkable biocompatible material; d) forming stable, preferably porous capsules from cross-linked biocompatible material, which capsules contain incorporated adherent cells; e) proliferating the adherent cells in the capsules for a specified time period; f) breaking up the capsule material by means of a physical or chemical stimulus and releasing the cells as a cell suspension. In an especially preferred embodiment of the invention, the method is performed cyclically, in that the cells released in step f) are suspended anew in a cell culture medium that contains a cross-linkable, biocompatible material or in a medium that contains at least one component that is required for the cross-linking of the cross-linkable, biocompatible material and steps c)-f) are repeated at least once.
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