The quality of staining histological specimens has an impact on the histological assessment of the specimen. One of the causes for variability in the accuracy is the variations in the staining of the specimens to be tested in the working laboratories. The present invention provides a method for assessing the staining quality in a working laboratory and providing a quantitative quality measure for the staining quality. Thereby, it is possible for each working laboratory to evaluate their staining quality and to get feedback of how to improve the quality. The invention further relates to a system and a software program for carrying out the method.