A smart toilet includes: a toilet body; a urine cup disposed outside the toilet body; a urine collection device disposed in the toilet body, including a rotation shaft and a front urine storage cup, a middle urine storage cup and a rear urine storage cup distributed around the rotation shaft, the front urine storage cup is provided with a first urine detection sensor and a first valve, the middle urine storage cup is provided with a second urine detection sensor and a second valve; a drive motor, connected with the rotation shaft; a controller, configured to control the drive motor to drive the rear urine storage cup to rotate to the urine collection position and control the second valve to be turned on upon urine volume in the middle storage cup reaching a volume threshold. An operation control method and device of a smart toilet are also provided.