Disclosed are a display substrate and a driving method thereof, and a display device. The display substrate includes a plurality of pixels, a data signal chip and a control unit. Each of the pixels includes a white sub-pixel; the data signal chip is for transmitting a set of data signals to the pixels, the set of data signals including data signals transmitted to the white sub-pixel; and the control unit controls the white sub-pixel to be in a dark state when the data signal chip transmits a predetermined set of data signals to the pixels. When the data signal chip transmits the predetermined set of data signals to the pixels, the display substrate controls the white sub-pixel to be in a dark state.