An interference fringe pattern (ILR) between an inline spherical wave light (L) and an off-axis reference light (R) is recorded with a photo detector (4), and on which spatial-frequency filtering is applied to obtain a complex amplitude in-line hologram (JLR). A complex amplitude off-axis hologram (JOR) is derived by performing a spatial frequency filtering on a hologram (IOR) in which an object light (O) emitted from a microscopic subject illuminated with a spherical wave light (L) is recorded with a reference light (R), and the derived data is divided with data of the hologram (JLR) so that a complex amplitude in-line hologram (JOL) from which a component of the reference light (R) is eliminated is generated and recorded.