A manufacturing device of a membrane-electrode assembly for fuel cell includes a membrane unwinder unwinding and supplying a polymer electrolyte membrane of a roll shape; a film unwinder unwinding and supplying a release film of a roll shape respectively coated with an anode catalyst electrode layer and a cathode catalyst electrode layer with a predetermined interval in an upper and lower sides of the polymer electrolyte membrane; upper and lower bonding rolls respectively disposed at the upper and lower sides of a progressing path of the polymer electrolyte membrane and the release film and pressed to an upper surface and a lower surface of the polymer electrolyte membrane; and a protection film unwinder unwinding and supplying a protection film between adhered surfaces of the release film and the upper and lower bonding rolls.