The present invention provides a light guide plate, a backlight module, and a method for manufacturing the light guide plate. The light guide plate include a light guide plate body, on which at least two layers of dots having a refractive index different from the light guide plate body are distributed. According to the light guide plate of the present invention, the dots are distributed on at least two layers, rather than merely on a single interface as that in the traditional light guide plate. As a result, the dot density in an identical region of the light guide plate will exceed the limit of 100%, and thereby a better light guide effect will be achieved. In addition, according to the method of the present invention, the light guide plate is manufactured based on a 3D printing technology.