Invention Grant
- Patent Title: Stabilized recombinant expression plasmid vector in hafnia alvei and applications thereof
-
Application No.: US14992412Application Date: 2016-01-11
-
Publication No.: US10221407B2Publication Date: 2019-03-05
- Inventor: Zhenhua Pang , Naiqiang Li , Charlie Liu , Xiucai Liu
- Applicant: CATHAY INDUSTRIAL BIOTECH LTD. , CATHAY R&D CENTER CO., LTD.
- Applicant Address: CN Shanghai CN Shanghai
- Assignee: Cathay Industrial Biotech Ltd.,Cathay R&D Center Co., Ltd.
- Current Assignee: Cathay Industrial Biotech Ltd.,Cathay R&D Center Co., Ltd.
- Current Assignee Address: CN Shanghai CN Shanghai
- Agency: Perkins Coie LLP
- Agent Yingli Wang
- Main IPC: C12N9/88
- IPC: C12N9/88 ; C12N15/74 ; C07C211/09 ; C12P13/00 ; C08G69/26

Abstract:
One aspect of the present disclosure relates to a stabilized recombinant expression plasmid vector comprising a polynucleotide encoding an antitoxin gene which expresses a polypeptide that neutralizes a polypeptide toxic to a host cell, the toxic polypeptide being expressed by a toxin gene in the host cell, and a polynucleotide encoding a polypeptide expression product, and the stabilized recombinant expression plasmid vector is derived from a Hafnia alvei autonomously replicable backbone plasmid. Other aspects of the present disclosure relate to a transformant transformed with the stabilized recombinant expression plasmid vector disclosed herein, a method of producing biobased cadaverine using the transformant disclosed herein, and biobased cadaverine prepared by the method disclosed herein. Another aspect of the present disclosure relates to a polyamide formed using biobased cadaverine disclosed herein, and a composition thereof. Another aspect of the present disclosure relates to a method of preparing 1,5-diisocyanatopentane comprising preparing biobased cadaverine using the method disclosed herein and converting the biobased cadaverine to 1,5-diisocyanatopentane.
Public/Granted literature
- US20160201048A1 STABILIZED RECOMBINANT EXPRESSION PLASMID VECTOR IN HAFNIA ALVEI AND APPLICATIONS THEREOF Public/Granted day:2016-07-14
Information query