Invention Grant
- Patent Title: Highly active mutant enzyme for producing 4-hydroxybenzoic acid or salt thereof
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Application No.: US15309332Application Date: 2015-05-13
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Publication No.: US10221409B2Publication Date: 2019-03-05
- Inventor: Hideaki Yukawa , Masayuki Inui , Kazumi Hiraga , Masako Suda , Ryoma Hashimoto
- Applicant: GREEN PHENOL DEVELOPMENT CO., LTD.
- Applicant Address: JP Kyoto
- Assignee: GREEN CHEMICALS CO., LTD.
- Current Assignee: GREEN CHEMICALS CO., LTD.
- Current Assignee Address: JP Kyoto
- Agency: Wenderoth, Lind & Ponack, L.L.P.
- Priority: JP2014-100875 20140514
- International Application: PCT/JP2015/063738 WO 20150513
- International Announcement: WO2015/174446 WO 20151119
- Main IPC: C12N9/88
- IPC: C12N9/88 ; C12N15/09 ; C12P7/42 ; C12N15/77

Abstract:
The mutant chorismate-pyruvate lyase (A) or (B) as described below is capable of producing 4-hydroxybenzoic acid or a salt thereof with sufficient practical efficiency.(A) A mutant chorismate-pyruvate lyase obtained by replacing the valine at position 80 in a chorismate-pyruvate lyase (ubiC) from Pantoea ananatis consisting of the amino acid sequence of SEQ ID NO: 1 with one or more other amino acids.(B) A mutant chorismate-pyruvate lyase obtained by replacing an amino acid in another chorismate-pyruvate lyase, the amino acid being at a position enzymologically homologous with that of the above valine, with one or more other amino acids.
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