Invention Grant
- Patent Title: Sensors, methods and kits for detecting nicotinamide adenine dinucleotides
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Application No.: US15550239Application Date: 2016-02-16
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Publication No.: US10221439B2Publication Date: 2019-03-05
- Inventor: Olivier Sallin , Luc Reymond , Kai Peter Johnsson
- Applicant: Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.
- Applicant Address: DE München
- Assignee: Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.
- Current Assignee: Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.
- Current Assignee Address: DE München
- Agency: Arent Fox LLP
- Priority: EP15155272 20150216
- International Application: PCT/EP2016/053280 WO 20160216
- International Announcement: WO2016/131833 WO 20160825
- Main IPC: C12Q1/00
- IPC: C12Q1/00 ; C12Q1/26 ; G01N33/573 ; G01N33/542
Abstract:
The invention relates to the in vitro and in cellulo detection of the cofactors nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucleotide phosphate (NADP). Provided is a sensor molecule for fluorescence- or luminescence-based detection of a nicotinamide adenine dinucleotide analyte, in particular for detecting the concentrations of NAD+, NADP+ and/or the ratios of the concentrations of NAD+/NADH and NADP+/NADPH, the sensor comprising (i) a binding protein (BP) for the nicotinamide adenine dinucleotide analyte, the BP being derived from sepiapterin reductase (SPR; EC 1.1.1.153) (ii) an SPR ligand (SPR-L) capable of intramolecular binding to said BP in the presence of the oxidized form of said analyte; and (iii) at least one fluorophore.
Public/Granted literature
- US20180087083A1 Sensors, Methods and Kits for Detecting Nicotinamide Adenine Dinucleotides Public/Granted day:2018-03-29
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