The present disclosure describes apparatuses and techniques for room temperature three-dimensional (3D) printing using edible printing materials. In some aspects, a 3D printer includes a printing platform configured to receive the extruded edible printing material from the printing cartridge, a cartridge receiver configured to hold an integrated printing cartridge that contains an edible printing material that can be extruded at room temperature, and a printing target. The position of the printing platform can be adjusted vertically and the position of the cartridge receiver can be adjusted in a plane perpendicular to the printing platform. The printing target is configured to allow extrusion of the edible printing material at precise and repeatable locations.