Sterilization machine for reprocessing an endoscope comprising a fixed part and a mobile part. The fixed part comprises a first frame and a first fluid-dynamic circuit for feeding and/or discharging fluids, and provided with a plurality of first connectors. The mobile part comprises a second frame on which a tank is mounted, a lid to hermetically close the tank and a second fluid-dynamic circuit connectable to the endoscope, able to be selectively coupled removably with the first connectors to achieve the fluidic communication with the first fluid-dynamic circuit. The second frame is provided with rolling means, and the first frame and the second frame comprise guide means and anchoring means to removably anchor the first frame to the second frame.