Invention Grant
- Patent Title: Synthetic oplophorus luciferases with enhanced light output
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Application No.: US15714210Application Date: 2017-09-25
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Publication No.: US10233485B2Publication Date: 2019-03-19
- Inventor: Lance P. Encell , Mary Hall , Paul Otto , Gediminas Vidugiris , Keith V. Wood , Monika G. Wood , Kristopher Zimmerman
- Applicant: Promega Corporation
- Applicant Address: US WI Madison
- Assignee: PROMEGA CORPORATION
- Current Assignee: PROMEGA CORPORATION
- Current Assignee Address: US WI Madison
- Agency: Casimir Jones, S.C.
- Agent David W. Staple
- Main IPC: C12Q1/66
- IPC: C12Q1/66 ; C12N9/00 ; C12N9/02
Abstract:
A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type Oplophorus luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type Oplophorus luciferase of SEQ ID NO:1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type Oplophorus luciferase.
Public/Granted literature
- US20180051318A1 SYNTHETIC OPLOPHORUS LUCIFERASES WITH ENHANCED LIGHT OUTPUT Public/Granted day:2018-02-22
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