An optical termination box comprising: a tubular housing (10) with an open end (10b) and a closed end (10a) provided with an input opening (11) for an optical distribution cable (100); a cover (20) securable against the open end (10b) of the housing (10) and pierced by output adapters (30); and an optical fiber accommodating tray (40), internal to the tubular housing (10) and having a first end (41) carrying a tubular bushing (50) with a base portion (51) seated against the closed end (10a) of the tubular housing (10), and a projecting body portion (52) out of the input opening (11), to cooperate with a puller (60) that holds the tubular bushing (50) hermetically pressed against the closed end (10a) of the tubular housing (10). The tubular bushing (50) hermetically receives and retains an end portion (100a) of an optical distribution cable (100) containing at least one optical fiber (FO).