An image quality is improved although a medical stereomicroscope optical system and a medical observation apparatus are small and light. An objective optical system and a plurality of imaging optical systems are arranged in an order from an object side to an image side, and the imaging optical system has at least a single aspheric surface. Accordingly, a spherical aberration and a field curvature are improved, and the image quality is improved although the medical stereomicroscope optical system and a medical observation apparatus are small and light.