Implementations of piezoelectric operated microscope focusing devices may include: a first block, having a first side and a second side, the first side coupled to a body of a microscope through four or more screws. The device may also include a second block, having a first surface, a second surface, and a side surface. The side surface may be coupled to the second side of the first block. The second surface of the second block may be configured to couple with an objective of the microscope. A piezoelectric operated microscope focusing device may also include an opening on the first surface of the second block. The opening may be configured to couple to an illuminator of a microscope. The opening may have a substantially rounded portion and an adapter orientated substantially perpendicular to the first block of the device. The adapter may include dove tail projections and mounting screws.