An analysis method of lane stripe images, an image analysis device and a non-transitory computer readable medium thereof are provided to perform steps of: setting a reference point as a center to recognize the lane stripe image in a plurality of default directions; defining a plurality of preset sections onto the lane stripe image and determining a characteristic value of the lane stripe image in each of the preset sections whenever the lane stripe image is recognized in one of the default directions; determining a first feature parameter according to the characteristic values of the lane stripe image in the preset sections when the lane stripe image is recognized in at least one of the default directions; and determining an actual lane parameter of the lane stripe image according to at least the first feature parameter.