A lens barrel includes: fixed barrel including straight groove extending in optical axis direction; cam ring rotatable around optical axis relative to the fixed barrel, and provided with first and second cam grooves; first barrel to which sub-barrel to hold first optic is detachably fixed, the first barrel including first cam follower slidably contacting with the straight groove and the first cam groove; second barrel holding second optic, and including second cam follower slidably contacting with the straight groove and the second cam groove; elastic member disposed between the first and second barrels when sub-barrel is fixed to the first barrel, and biasing the first and second barrels oppositely in the optical axis direction; and regulating mechanism regulating position of end of the elastic member relative to the first barrel without fixing the elastic member to the sub-barrel when the sub-barrel is fixed to the first barrel.