Invention Grant
- Patent Title: Methods of culturing retinal pigmented epithelium cells, including Xeno-free production, RPE enrichment, and cryopreservation
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Application No.: US15816912Application Date: 2017-11-17
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Publication No.: US10246682B2Publication Date: 2019-04-02
- Inventor: Dennis Clegg , Sherry T. Hikita , Lincoln V. Johnson , Liane Miller , Marlene Tsie , Chelsea D. Presbrey , Lisa R. Conti , Michelle A. Maloney , David R. Hinton , Qirui Hu
- Applicant: The Regents of the University of California
- Applicant Address: US CA Oakland US CA Los Angeles
- Assignee: The Regents of the University of California,University of Southern California
- Current Assignee: The Regents of the University of California,University of Southern California
- Current Assignee Address: US CA Oakland US CA Los Angeles
- Agency: Kilpatrick Townsend & Stockton LLP
- Main IPC: C12N5/02
- IPC: C12N5/02 ; C12N5/00 ; C12N5/10 ; C12N5/071 ; C12N15/00 ; C12N5/079
Abstract:
The production of high quality retinal pigmented epithelium (RPE) cells is necessary for research and potential therapeutic uses. Especially desirable are methods for the production of RPE cells using xeno-free culture conditions. Disclosed herein are novel methods for the production of RPE cells from pluripotent cells with high yields, including xeno-free production methods. Also provided are methods of efficiently isolating RPE cells from cultures containing heterogeneous cell types, allowing for substantially pure RPE cell cultures to be established. Additionally, novel methods for the cryopreservation of RPE cells are provided.
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