Invention Grant
- Patent Title: Selective amplification of desired nucleic acid regions in a target sequence
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Application No.: US16063227Application Date: 2017-01-27
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Publication No.: US10246740B2Publication Date: 2019-04-02
- Inventor: Ulf Klangby , Anders Hedrum , Steffen Heim
- Applicant: Devyser Holding AB
- Applicant Address: SE Hägersten
- Assignee: Devyser Holding AB
- Current Assignee: Devyser Holding AB
- Current Assignee Address: SE Hägersten
- Agency: Bozicevic, Field & Francis LLP
- Agent James S. Keddie
- Priority: EP16152951 20160127
- International Application: PCT/EP2017/051793 WO 20170127
- International Announcement: WO2017/129756 WO 20170803
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12Q1/686 ; C12Q1/6848
Abstract:
A method and kit for selective amplification of desired amplicons in a two-stage polymerase chain reaction is provided. The method and kit uses target primers comprising mismatch control sequences; wherein any pair of primers that flank a desired amplicon sequence have non-matching mismatch control sequences. and any pair of primers that flank an undesired amplicon sequence have matching mismatch control sequences.
Public/Granted literature
- US20180371528A1 SELECTIVE AMPLIFICATION OF DESIRED NUCLEIC ACID REGIONS IN A TARGET SEQUENCE Public/Granted day:2018-12-27
Information query
