The present disclosure provides a pixel unit, an array substrate and a manufacturing method thereof. The pixel unit comprises slit electrodes and comprises four zones. The slit electrodes in the four zones are electrically connected with each other. The slit incline directions of the slit electrodes of the pixel unit in each of the four zones are the same, and the slit inclined directions of the slit electrodes of the pixel unit in any two adjacent zones of the four zones are different. The pixel unit structure with a plurality of domains and the array substrate comprising the pixel unit structure provided by the present disclosure can eliminate color deviation to the greatest extent, and have excellent light transmittance.