An intelligent storage device signal transmission method includes steps of: electrically connecting intelligent storage module and electronic device; enabling built-in identification system of intelligent storage module uses numeric identification code to identify system type of electronic device and enabling USB communication protocol channel for signal transmission between intelligent storage module and electronic device so that file system of downloaded APP of electronic device can identify file format of intelligent storage module and microprocessor unit of intelligent storage module can define data packet format of DATA block and then transmit data packet to APP of electronic device for data packet resolution. Thus, built-in file system of App of electronic device can identify and solve received data packet and provide file data of resolved data file to data storage software of electronic device for application; and electronic connection between intelligent storage module and electronic device is disconnected after signal transmission is completed.