Invention Grant
- Patent Title: Method for producing ordered protein lattice
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Application No.: US14913038Application Date: 2014-08-21
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Publication No.: US10253302B2Publication Date: 2019-04-09
- Inventor: John Charles Sinclair , Ceri John Lewis
- Applicant: Crysalin Ltd.
- Applicant Address: GB London, Greater London
- Assignee: Crysalin Ltd.
- Current Assignee: Crysalin Ltd.
- Current Assignee Address: GB London, Greater London
- Agency: Hamilton, Brook, Smith & Reynolds, P.C.
- Priority: GB1314945.5 20130821
- International Application: PCT/GB2014/052565 WO 20140821
- International Announcement: WO2015/025167 WO 20150226
- Main IPC: C12N9/04
- IPC: C12N9/04 ; C07K1/30 ; B82Y5/00

Abstract:
A method for producing an ordered protein lattice, the method comprising: (a) providing a first component comprising a subunit of a homooligomeric protein assembly fused to a first subunit of a heterooligomeric protein assembly, and a second component comprising a second subunit of the heterooligomeric protein assembly, wherein the homooligomeric protein assembly and the heterooligomeric protein assembly are each symmetrical in two or three dimensions and share a rotational symmetry axis of the same order; (b) mixing said first monomer and said second monomer to produce a mixture; and (c1) (i) heating the mixture to a temperature about 2° C. to about 10° C. below the visible dissociation temperature; (ii) cooling the mixture by about 10° C. to about 20° C.; and (iii) repeating steps (i) and (ii) at least 10 times; or (c2) (i) heating the mixture to a temperature about 2° C. to about 30° C. or more below the visible dissociation temperature; and (ii) holding the mixture at a temperature about 2° C. to about 30° C. or more below the melt temperature, thereby producing an ordered protein lattice.
Public/Granted literature
- US20160194614A1 Protein Crystals Public/Granted day:2016-07-07
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