Invention Grant
- Patent Title: Method for transfection of nucleic acids into eukaryotic cells in 3D scaffold
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Application No.: US14556751Application Date: 2014-12-01
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Publication No.: US10253315B2Publication Date: 2019-04-09
- Inventor: Jonathan Bruniaux , Xavier Gidrol , Fabrice Navarro Y Garcia , Eric Sulpice
- Applicant: Commissariat à l'énergie atomique et aux énergies alternatives
- Applicant Address: FR Paris
- Assignee: COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES
- Current Assignee: COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES
- Current Assignee Address: FR Paris
- Agency: Stites & Harbison, PLLC
- Agent B. Aaron Schulman, Esq.
- Priority: FR1258115 20120830; FR1261520 20121130
- Main IPC: C12N15/10
- IPC: C12N15/10 ; C12N5/071 ; C12N15/64 ; C12Q1/68 ; C12N5/09 ; C12N15/87 ; B82Y5/00
Abstract:
The present invention concerns a method for in vitro or ex vivo transfection of a nucleic acid into a eukaryotic cell in 3D scaffold by contacting eukaryotic cells in 3D scaffold with a formulation in nanoemulsion form which comprises a continuous aqueous phase and a least one dispersed phase, and which comprises said nucleic acid. The invention further relates to a method of screening implementing said method of transfection, and to 3D scaffold comprising eukaryotic cells and said formulation in nanoemulsion form which comprises a continuous aqueous phase and a least one dispersed phase, and which comprises said nucleic acid.
Public/Granted literature
- US20150159153A1 METHOD FOR TRANSFECTION OF NUCLEIC ACIDS INTO EUKARYOTIC CELLS IN 3D SCAFFOLD Public/Granted day:2015-06-11
Information query
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