Invention Grant
- Patent Title: Mutant sugar isomerase with improved activity, derived from E. coli, and production of L-gulose using the same
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Application No.: US15318172Application Date: 2014-06-13
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Publication No.: US10253341B2Publication Date: 2019-04-09
- Inventor: Jung-Kul Lee , Chandra Sujan Sigdel , Jinglin Li , Tae-Su Kim
- Applicant: KONKUK UNIVERSITY INDUSTRIAL COOPERATION CORP.
- Applicant Address: KR Seoul
- Assignee: KONKUK UNIVERSITY INDUSTRIAL COOPERATION CORP.
- Current Assignee: KONKUK UNIVERSITY INDUSTRIAL COOPERATION CORP.
- Current Assignee Address: KR Seoul
- Agency: Baker & Hostetler LLP
- Priority: KR10-2014-0071473 20140612
- International Application: PCT/KR2014/005218 WO 20140613
- International Announcement: WO2015/190633 WO 20151217
- Main IPC: C12N9/90
- IPC: C12N9/90 ; C12P19/02 ; C12P19/24
Abstract:
The present invention relates to improving the enzymatic activity of Escherichia coli O157:H7-derived sugar isomerase by molecular mechanics and site-directed mutagenesis. More particularly, the present invention relates to a sugar isomerase having an enzymatic activity improved by mutation, a nucleic acid molecule encoding the same, a vector comprising the nucleic acid molecule, a transformant comprising the vector, a mutant of the sugar isomerase, and a method for producing L-gulose using the improved sugar isomerase.
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