Systems and methods for treating target tissues within the ears are disclosed. Systems include a device including a cutting edge configured to form an incision in a tympanic membrane, a pressure equalization tube positionable within an interior space of the device in a compressed configuration and configured to expand into an expanded configuration when released from the interior space, and an elongate positioning element configured to release the pressure equalization tube from the interior space such that the pressure equalization tube is deployed in the incision formed in the tympanic membrane.