An apparatus for growing and separating cells or micro carriers from fluid medium in an aseptic environment, has a sealed, air and liquid tight, container having a port to allow for the introduction of fluid, gas or components, a filter with a filtration medium mounted centrally around an axis extending through the container, a mixing assembly that rotates around the filtration medium to allow for suspension of media or agitation thereof, and the mixing assembly has vanes containing a magnetic unit in a lower portion thereof that allows for coupling to a stir plate for driving the vanes of the mixing assembly. The vanes are preferably suspended by a central bearing located above the filter, and preferably the vanes extend downward to proximate the bottom of the container to the same extent as the filter and a diptube located along the axis of the filter and vanes.