• Patent Title: Diacylgylcerol acyl transferase (DGA1) polynucleotides, and methods of increasing yeast cell lipid production by overexpression of heterologous DGA1
  • Application No.: US15120952
    Application Date: 2015-02-24
  • Publication No.: US10260052B2
    Publication Date: 2019-04-16
  • Inventor: Elena E. BrevnovaArthur J. Shaw, IVEmily H. Greenhagen
  • Applicant: Novogy, Inc.
  • Applicant Address: US MA Cambridge
  • Assignee: NOVOGY, INC.
  • Current Assignee: NOVOGY, INC.
  • Current Assignee Address: US MA Cambridge
  • Agent Albert Shung
  • International Application: PCT/US2015/017227 WO 20150224
  • International Announcement: WO2015/127421 WO 20150827
  • Main IPC: C12N1/20
  • IPC: C12N1/20 C12N9/10 C12P7/64
Diacylgylcerol acyl transferase (DGA1) polynucleotides, and methods of increasing yeast cell lipid production by overexpression of heterologous DGA1
Abstract:
DGA1 catalyzes the final enzymatic step for converting acyl-CoA and 1,2-diacylglycerol to triacylglycerols (TAG) and CoA in yeast. Disclosed are methods for expression in an oleaginous yeast host of polynucleotide sequences encoding DGA1 from Rhodosporidium toruloides, Lipomyces starkeyi, Aurantiochytrium limacinium, Aspergillus terreus, or Claviceps purpurea. Also described herein are engineered recombinant host cells of Yarrowia lipolytica comprising heterologous DGA1 polynucleotides encoding DGA1 proteins, or functionally active portions thereof, having the capability of producing increased lipid production and possessing the characteristic of enhanced glucose consumption efficiency.
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