Invention Grant
- Patent Title: Accelerated isothermal amplification of DNA
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Application No.: US15213194Application Date: 2016-07-18
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Publication No.: US10260090B2Publication Date: 2019-04-16
- Inventor: Rhett L. Martineau , Shih-hui Chao , Weimin Gao , Shufang Ci , Deirdre R. Meldrum
- Applicant: Rhett L. Martineau , Shih-hui Chao , Weimin Gao , Shufang Ci , Deirdre R. Meldrum
- Applicant Address: US AZ Scottsdale
- Assignee: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY
- Current Assignee: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY
- Current Assignee Address: US AZ Scottsdale
- Agency: Lee & Lin IP PLLC
- Main IPC: C12P19/34
- IPC: C12P19/34 ; C12Q1/68 ; C12Q1/6844

Abstract:
A method of synthesizing a nucleic acid complementary to a target nucleic acid sequence in a template nucleic acid includes annealing a swarm primer to a target nucleic acid, the swarm primer overlapping an F1 site of the target nucleic acid and extends toward the F2 site of the target nucleic acid. An inner primer may also be annealed to the target nucleic acid to produce a complimentary nucleic acid having a single-strand loop onto which further primers may anneal. A plurality of amplicons may be reproduced, many of which may have further primers annealed thereto to generate more complementary nucleic acids.
Public/Granted literature
- US20170107549A1 ACCELERATED ISOTHERMAL AMPLIFICATION OF DNA Public/Granted day:2017-04-20
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