Larvicide and pupicide formulation and methods of administration thereof, harmless for humans and other animals, able to kill all the instars larval stages and pupae in about two hours and fifteen minutes, respectively, with no impact on the environment. Larvicide/pupicide formulation and methods of administration thereof for killing larvae and pupae of Anopheles gambiae and An. stephensi, main vectors of malaria, and Aedes aegypti and Ae. albopictus, main vectors of dengue, Zika virus, chikungunya and yellow fever.