The present invention discloses a method for the preparation of skipjack tuna extract having hypouricemic effect and the use thereof, which is simple in processing operations, low in production cost, and is free from pollution. The prepared skipjack tuna extract has a potent hypouricemic effect, and has a significant therapeutic effect on hyperuricemia, with no toxic and side effects. The method in an example of the present invention comprises: pretreating skipjack tuna to obtain a skipjack tuna slurry; enzymolysing the skipjack tuna slurry to obtain a crude enzymolysis liquid; removing fishy smell and bitter taste, removing impurities by activated charcoal, and filtering to obtain a refinded enzymolysis liquid; concentrating under vacuum and spray-drying to obtain the skipjack tuna extract. The present invention further discloses use of the skipjack tuna extract in health care products or food products.