Invention Grant
- Patent Title: Mutant enzyme and production method for terpenoid using said mutant enzyme
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Application No.: US15302903Application Date: 2015-04-09
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Publication No.: US10280406B2Publication Date: 2019-05-07
- Inventor: Toshihiko Takehana , Seiji Koike , Tomohisa Kuzuyama
- Applicant: ADEKA CORPORATION
- Applicant Address: JP Tokyo
- Assignee: ADEKA CORPORATION
- Current Assignee: ADEKA CORPORATION
- Current Assignee Address: JP Tokyo
- Agency: Heslin Rothenberg Farley & Mesiti P.C.
- Priority: JP2014-080242 20140409
- International Application: PCT/JP2015/061168 WO 20150409
- International Announcement: WO2015/156369 WO 20151015
- Main IPC: C12N9/04
- IPC: C12N9/04 ; C12P5/00 ; C12N15/52
Abstract:
The object of the present invention is to efficiently produce useful terpenoid compounds, and specifically, to provide a method for preparing squalene, which is an important intermediate of terpenoid. The object can be solved by a hydroxymethylglutaryl CoA reductase (HMGR) comprising: (a) an amino acid other than alanine (A) at the 10th position in an Sα2 amino acid sequence of HMGR, (b) an amino acid other than proline (P) at the 1st position from the carboxyl terminal in the DKK region of the HMG-CoA binding site of HMGR, (c) an amino acid other than alanine (A) at the 1st position in an Lα2 amino acid sequence of HMGR, and (d) an amino acid other than glutamic acid (E) at the 6th position in an Lα2 amino acid sequence of HMGR of the present invention.
Public/Granted literature
- US20170088824A1 MUTANT ENZYME AND PRODUCTION METHOD FOR TERPENOID USING SAID MUTANT ENZYME Public/Granted day:2017-03-30
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