A light guide plate and a backlight module are described. The light guide plate includes a main body and a plurality of stripe microstructures. The main body includes a light incidence surface, a light-emitting surface and a light reflective surface. The light-emitting surface is opposite to the light reflective surface, and the light incidence surface is connected between the light-emitting surface and the light reflective surface. The light-emitting surface includes a microstructure region adjacent to the light incidence surface. The stripe microstructures are arranged in the microstructure region, and parallel to a normal line of the light incidence surface.