A computer chassis capable of receiving multiple motherboards includes a shell and a first motherboard received in the shell. The shell includes a plurality of grooves and a pressing column. The computer chassis further includes a motherboard module, the motherboard module includes a bracket and a second motherboard received in the bracket. The bracket includes an operation member and a plurality of guide rails. The guide rails are inserted into the grooves, the operation member is rotated to drive the bracket to the first motherboard, until the motherboard module is slidably rotated in the shell. The second motherboard is thereby electrically connected with the first motherboard.