An operating method of a memory controller includes steps of: configuring the memory controller to receive a read command and read at least one piece of first data stored in a non-volatile memory according to the received read command; configuring the memory controller to determine whether a read count of the at least one piece of first data is greater than a set value; and configuring the memory controller to copy and store the at least one piece of first data in a data temporary storage device when the read count of the at least one piece of first data is determined to be greater than the set value. A data storage device and another operating method are also provided.