A method of artificially inseminating a hen includes the steps of securing a male bird, applying a semen extender liquid to a tip of a cloacal protuberance of the male bird, squeezing the cloacal protuberance to extract semen from the male bird into the semen extender liquid to form a mixture, applying a tip of a pipette to the mixture, drawing the mixture into the pipette, securing the hen, inserting the pipette into the hen's cloaca, and squeezing a bulb of the pipette to inject the mixture from the pipette into the cloaca. A holding device for restraining a bird during sperm collection and artificial insemination procedures has a base, an arm extending upward from the base, and an open-ended receptacle connected to the upper end of the arm. The holding device is useful in mitigating injury to the bird and the handler during the procedures.