Invention Grant
- Patent Title: Butyrate-producing bacterium and use thereof
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Application No.: US15129849Application Date: 2015-03-27
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Publication No.: US10293004B2Publication Date: 2019-05-21
- Inventor: Tadashi Sato , Shiro Kusuhara , Wakae Yokoi , Masahiko Ito , Kouji Miyazaki , Akira Kushiro
- Applicant: KABUSHIKI KAISHA YAKULT HONSHA
- Applicant Address: JP Minato-ku
- Assignee: KABUSHIKI KAISHA YAKULT HONSHA
- Current Assignee: KABUSHIKI KAISHA YAKULT HONSHA
- Current Assignee Address: JP Minato-ku
- Agency: Oblon, McClelland, Maier & Neustadt, L.L.P.
- Priority: JP2014-067726 20140328
- International Application: PCT/JP2015/059670 WO 20150327
- International Announcement: WO2015/147277 WO 20151001
- Main IPC: A61K45/00
- IPC: A61K45/00 ; A61K47/00 ; A01N63/00 ; A01N65/00 ; A61K35/74 ; C12N1/20 ; C12P7/52 ; C12R1/01 ; A23K10/18 ; A23K20/00 ; A23K20/105 ; A23K20/163 ; A23L33/10 ; A23L33/135 ; A23L33/21 ; A61K31/047 ; A61K31/7004 ; A61K31/7016 ; A61K31/702 ; A61K31/19 ; A61K47/26 ; C12N15/09

Abstract:
A butyrate-producing bacterium belonging to Anaerostipes hadrus is provided. The amount of butyrate produced is at least 1.5 times that of Anaerostipes hadrus YIT 10092T (DSM 3319T) and is measured by thawing a frozen stock solution of the bacterial strain (a 10% (w/v) skim milk-2% sodium glutamate solution with suspended bacterial cells) (cell count: 2.0 to 5.5×1010 cells/mL), inoculating the solution at 1% to 4 mL of a PY liquid medium supplemented with 33 mM sodium acetate and 0.5 (w/v) % glucose (PYGA medium), followed by anaerobic culture at 37° C. for 24 hours, then inoculating the culture solution at 1% to a PYGA medium, followed by anaerobic culture at 37° C. for 24 hours, then inoculating the culture solution at 1% to a PY medium containing 33 mM sodium acetate and 0.5 (w/v) % L-sorbose, followed by anaerobic culture at 37° C. for 24 hours, and then measuring the butyrate concentration.
Public/Granted literature
- US20170246220A1 BUTYRATE-PRODUCING BACTERIUM AND USE THEREOF Public/Granted day:2017-08-31
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