- Patent Title: Temporal focusing-based multiphoton excitation fluorescence microscopy system capable of tunable-wavelength excitation and excitation wavelength selection module thereof
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Application No.: US15897446Application Date: 2018-02-15
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Publication No.: US10295469B2Publication Date: 2019-05-21
- Inventor: Fan-Ching Chien , Chi-Hsiang Lien
- Applicant: NATIONAL CENTRAL UNIVERSITY
- Applicant Address: TW Taoyuan
- Assignee: NATIONAL CENTRAL UNIVERSITY
- Current Assignee: NATIONAL CENTRAL UNIVERSITY
- Current Assignee Address: TW Taoyuan
- Agency: Marquez IP Law Office, PLLC
- Agent Juan Carlos A. Marquez
- Priority: TW106116835A 20170522
- Main IPC: G01N21/64
- IPC: G01N21/64 ; G02B5/18 ; G02B21/00 ; G02B27/10

Abstract:
The invention provides a temporal focusing-based multiphoton excitation fluorescence microscopy system capable of tunable-wavelength excitation and an excitation wavelength selection module thereof. The temporal focusing-based multiphoton excitation fluorescence microscopy system comprises: an excitation light generating module for generating excitation light; an excitation wavelength selection module for generating reflected light having a predetermined output angle in accordance with the wavelength of the excitation light and generating detecting excitation light through a diffraction unit; and a fluorescent microscope. With the practice of the present invention, the wavelength of the excitation light can be changed by the excitation wavelength selection module in real time to excite different fluorophores with the corresponding maximum multiphoton absorption wavelengths, so as to obtain better fluorescent signals and improve the image quality of the temporal focusing-based multiphoton excitation fluorescence microscope system.
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