A display substrate, display device and manufacturing method the same are provided. The display device includes an ordered porous thin film; a plurality of sub-pixel regions arranged in a matrix, a plurality of pore passages being disposed in each sub-pixel region, each pore passage having an opening on the surface of the ordered porous thin film; a plurality of quantum dots respectively arranged in at least part of the plurality of pore passages; and a first electrode layer and a second electrode layer respectively arranged on two sides of the ordered porous thin film, the second electrode layer including a plurality of sub-electrodes spaced apart from each other and respectively corresponding to the plurality of sub-pixel regions. The display substrate, display device and their manufacturing method have relatively high color gamut and quantum dots light-emitting efficiency.