Devices, systems, and methods of generating and managing behavioral biometric cookies. The system monitors user-interactions of a user, that are performed via an input unit of an end-user device; and extracts a set of user-specific characteristics, which are used as a behavioral profile or behavioral signature. The set of user-specific characteristics are further used as a behavioral biometric cookie data-item, allowing the system to distinguish between two human users that utilize the same electronic device; and allowing the system to distinguish between a human user and an automated script. The system further allows creation and utilization of behavioral sub-cookies that distinguish among multiple users of the same device. The system also allows creation of a cross-device behavioral cookie, to track browsing or usage history of a single user across multiple electronic devices.