A door opening and closing device for a refrigerator includes a door coupled by means of a hinge unit to a main body defining a storage compartment therein, a gasket disposed between the door and the main body to provide a hermetic seal therebetween, and an opening and closing unit for opening and closing the door with respect to the main body, wherein the opening and closing unit includes a drive unit for generating a driving force, a gasket separation mechanism for pushing the main body using rotational force transmitted from the drive unit to separate the gasket from the main body or the door, and a door rotating mechanism for rotating the door using the rotational force transmitted from the drive unit.