A positioning device with tolerance compensation including: a screw; a primary tightening member, and a secondary nut. The screw includes a driving portion cooperating with a driving tool to rotatably drive the screw and is located in an axial end portion of the screw; primary and secondary external threads, the primary external thread being further from the driving portion than the secondary external thread, the directions of the secondary external thread and the primary external thread are opposing; and an abutment projecting between the primary and secondary external threads. The primary tightening member includes a primary internal thread adapted to cooperate with the primary external thread, the primary tightening member and the abutment secured to an intermediate component. The secondary nut includes a secondary internal thread cooperating with the secondary external thread and is configured to bear against the intermediate component to fasten a first component on a second component.