The present application provides a liquid crystal panel structure containing a functionalized graphene layer and a method of preparing a functionalized graphene layer. In the liquid crystal panel structure containing a functionalized graphene layer of the present application, the functionalized graphene layer thereof simultaneously plays effects of transparent conductivity and liquid crystal alignment, an alignment process that subsequently uses an alignment material is not necessary, the fabrication process and film structures of the liquid crystal panel are greatly simplified. The method of preparing the functionalized graphene layer of the present application sufficiently utilizes superiority of transparent conductivity of the graphene, and modifiability of the graphene surface, to endow the graphene with an effect of liquid crystal molecule alignment, the functionalized graphene layer prepared thereby has transparency, conductivity and alignment function.