The present invention is directed to an Antimicrobial Disposable Seat Cover that is made of a material that is light, disposable and biodegradable, but resistant to dust mites, bed bugs, lice, mold, fungi, feces, viruses and bacteria, and a whole host of various microorganisms. Moreover, the provided Antimicrobial Disposable Seat Cover product is waterproof, breathable and as easy to use as a conventional disposable toilet seat cover. It will be AstM f1670 tested, and include an indicator of heat or moisture to indicate prior use. Any available pre-manufactured antimicrobial fabric or textile may be used in construction of the provided Antimicrobial Disposable Seat Cover product. Additionally, any fabric or textile that has been finished to exhibit antimicrobial characteristics may be used to configure and construct the present Antimicrobial Disposable Seat Covers provided herein.