Invention Grant
- Patent Title: Antibody gene expression-secretion system
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Application No.: US15308452Application Date: 2015-04-17
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Publication No.: US10344093B2Publication Date: 2019-07-09
- Inventor: Shun-ichiro Taniguchi , Yasuto Akiyama , Takeshi Masaki , Hitomi Shimizu
- Applicant: SHINSHU UNIVERSITY
- Applicant Address: JP Nagano
- Assignee: Shinshu University
- Current Assignee: Shinshu University
- Current Assignee Address: JP Nagano
- Agency: Occhiuti & Rohlicek LLP
- Priority: JP2014-095440 20140502
- International Application: PCT/JP2015/002133 WO 20150417
- International Announcement: WO2015/166641 WO 20151105
- Main IPC: C07K16/32
- IPC: C07K16/32 ; C07K16/28 ; A61K39/395 ; A61K35/74 ; C12N15/00 ; C12N15/09 ; C12P21/00

Abstract:
It is an object of the present invention to provide signal sequence information capable of secreting an antibody to the outside of cells in generation of the antibody by microorganisms of genus Bifidobacterium, and an antibody expression vector capable of secreting an antibody to the outside of cells by utilizing the signal sequence information. As a means for achieving the aforementioned object, there is prepared Bifidobacterium longum, which is transformed with a vector having inserted thereinto a DNA insert comprising the 5′-terminus of an antibody gene linked to the 3′-terminus of a DNA encoding a signal peptide-linker conjugate having a linker linked to the C-terminus of a signal peptide consisting of an amino acid sequence shown in SEQ ID NO: 1.
Public/Granted literature
- US20170218072A1 ANTIBODY GENE EXPRESSION-SECRETION SYSTEM Public/Granted day:2017-08-03
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