A method of determining the cetane number of a fuel in an internal combustion engine comprising, during running of the engine, i) with respect to one cylinder, performing a routine including a series of injections such that for each injection a quantity of fuel is injected into the cylinder, and during the routine varying the angle at which the injections takes place with respect to crankshaft angle; ii) measuring engine speed at intervals during the series of injections and determining values for changes in engine speed consequent to the injections; iii) determining cetane number from a pre-stored relationship relating the cetane number to changes in engine speed consequent to changes in the test injection angle.