Disclosed are a data transmission device for modulating the amplitude of a PAM-4 signal using a toggle serializer and a method of operating the same. In accordance with an embodiment of the present disclosure, the data transmission device includes a toggle serializer configured to generate at least one toggle signal by detecting logic level change of first and second signals from a Pulse Amplitude Modulation (PAM) signal including the first and second signals; and a driver configured to modulate an amplitude of the PAM signal by combining the first signal, the second signal, and the at least one toggle signal.